The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects.
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Bacterial luciferin may occur in free-living or symbiont bacteria (e.g., in squid such as Heteroteuthis dispar) or in fish such as Melanocetous johnsoni.
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